Optical constants of Phosphate Buffered Saline (PBS)
Barroso et al. 2019: DPBS; n 0.5–1.1 µm
Complex refractive index (n+ik)
Derived optical constants
Dispersion formula
$$n^2=1.7828-0.0015λ^{-2}+0.0009λ^{-4}-0.0179λ^{2}$$Conditions
temperature: 295
Comments
Dulbecco’s Phosphate Buffered Saline (DPBS, Sigma-Aldrich). 22 °C.
References
Á. Barroso, R. Radhakrishnan, S. Ketelhut, J. Schnekenburger, B. Kemper. Refractive index determination of buffer solutions from visible to near-infrared spectral range for multispectral quantitative phase imaging using a calibrated Abbe refractometer. Proc. SPIE 10887, 1088729 (2019)
Data
Additional information
About Phosphate buffered saline
Phosphate Buffered Saline (PBS) is a crucial buffer solution in biological and biochemical research, maintaining pH stability and osmotic balance. Composed of sodium chloride, sodium phosphate, and potassium chloride in distilled water, PBS is isotonic and mimics physiological conditions, making it ideal for cell culture, tissue preparation, and molecular biology applications. Its refractive index, typically around 1.334 at 20°C, ensures minimal light scattering, crucial for microscopy and spectroscopy techniques. PBS's precise formulation and optical properties support its widespread use in maintaining sample integrity and enhancing analytical sensitivity across various scientific disciplines.
Other names and variations:- PBS
- Dulbecco’s phosphate buffered saline
- DPBS