Optical constants of Basal Medium Eagle (BME, DMEM)
Barroso et al. 2019: DMEM; n 0.5–1.1 µm
Complex refractive index (n+ik)
Derived optical constants
Dispersion formula
$$n^2=1.7878-0.0020λ^{-2}+0.0011λ^{-4}-0.0188λ^{2}$$Conditions
temperature: 295
Comments
Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich) supplemented with 2 mM glutamine, 1 mM sodium pyruvate, and 1% penicillin-streptomycin. 22 °C.
References
Á. Barroso, R. Radhakrishnan, S. Ketelhut, J. Schnekenburger, B. Kemper. Refractive index determination of buffer solutions from visible to near-infrared spectral range for multispectral quantitative phase imaging using a calibrated Abbe refractometer. Proc. SPIE 10887, 1088729 (2019)
Data
Additional information
About Basal Medium Eagle
Basal Medium Eagle (BME) is a widely used cell culture medium designed to support the growth of various mammalian cells in vitro. Developed by Harry Eagle, it provides essential nutrients, including amino acids, vitamins, salts, and glucose, necessary for cell maintenance and proliferation. BME serves as the foundation for more complex media, such as Dulbecco’s Modified Eagle Medium (DMEM), which contains higher glucose concentrations and additional supplements to support more demanding cell lines. The refractive index of BME and DMEM solutions, typically around 1.335–1.340 at 20°C, is crucial for optical applications such as phase-contrast and fluorescence microscopy. These media play a fundamental role in biomedical research, including tissue engineering, drug development, and molecular biology studies.
Other names and variations:- BME
- Dulbecco’s Modified Eagle Medium
- DMEM